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. 2019 Nov 26;24(2):1319–1331. doi: 10.1111/jcmm.14789

Figure 6.

Figure 6

Inhibition of autophagy abrogated the migration and NF‐κB activation in CSE‐treated lung macrophages. A, After MH‐S cells were treated with 0.5% CSE for 0, 5, 10, 15 or 30 min, the level of p‐p65 (Ser536) was assessed by Western blot. B, MH‐S cells were pre‐treated with chicken IgY (5 μg/mL), anti‐HMGB1 (5 μg/mL) or Gly (100 nmol/L) for 90 min, followed by 0.5% CSE incubation for 15 min. The level of p65 phosphorylation was assessed by Western blot. C, MH‐S cells were pre‐treated with CQ (50 μmol/L) or 3‐MA (2 mmol/L) for 2 h, followed by 0.5% CSE incubation for 24 h. Migration assay was assessed. Bar: 100 μm. (D‐E) MH‐S cells were pre‐treated with CQ (50 μmol/L) or 3‐MA (2 mmol/L) for 2 h, followed by 0.5% CSE incubation for 15 min. The level of p‐p65 (Ser536) was assessed by Western blot. *P < .05 vs Control group. # P < .05. Values are mean ± SEM of three independent experiments