Fig 2. Analysis of irradiated PV.
A) and B) show western blot analysis of PV1-S and PV2-S samples (respectively) that were irradiated at the indicated doses of gamma radiation in the presence (+) or absence (-) of DP1 and MnCl2 (Mn) as shown. C) Electrophoresis of PCR products from PV2-S samples irradiated for the times indicated, +/- MDP. Viral RNA was extracted from samples following treatments and subject to RT-PCR. RT-PCR products were detected with a subsequent round of PCR and the resulting DNA fragments were resolved via electrophoresis and visualized using SYBR Safe DNA Gel Stain. DP1, decapeptide 1: DEHGTAVMLK.