Table 2. Evaluation of Ebola sGP detection kit analytical sensitivity in infected monkey cohort.

		Biological replicate 1			Biological replicate 2
Infection dose	Day post-infection	Test line (Delta OD)	Concentration (RNA Copy/mL)	Titer (FFU/mL)	Test line (Delta OD)	Concentration (RNA Copy/mL)	Titer (FFU/mL)
101	0	18	ND	ND	20	ND	ND
	2	17	ND	ND	0	ND	ND
	5	229	2.21 x 104*	5.20x 102	45	5.85 x 105	1.00 x 102
	7	283	1.14 x 109	1.05x 106	304	5.35 x 106	3.27 x 104
	9	246	1.87 x 108	5.35x 104	271	4.60 x 105	1.50x 102
102	0	6	ND	ND	10	ND	ND
	2	8	3.49 x 104*	ND	4	ND	ND
	5	301	8.00 x 107	8.20x 104	286	8.58 x 108	1.00 x 105
	7	252	3.66 x 109	1.40x 106	262	2.15 x 1010	2.02 x 106
	9	248	5.71 x 108	2.30x 104	177	7.17 x 109	1.00x 106
103	0	NT	NT	NT	2	ND	ND
	2	3	1.26 x 104*	ND	9	ND	ND
	5	311	2.59 x 108	1.16 x 105	93	4.43 x 106	1.00 x 101
	7	222	1.02 x 109	5.92 x 105	280	1.47 x 106	1.25 x 105
	9	273	2.36 x 108	5.97x 104	243	1.75 x 105	1.50x 103

Note: Infection dose (FFU/biological replicates); Test line (Ebola sGP Detection Kit test line delta OD positive values in bold characters); Concentration (mean of duplicates genome copies/ml), values noted *: samples with only one replicate positive out of two; Viral titer (FFU/ml); ND: not detected, NT: not tested.