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. 2020 Jan 29;5(1):e00550-19. doi: 10.1128/mSphere.00550-19

FIG 1.

FIG 1

Effect of T. gondii infection on HUVEC barrier function. (A) Transendothelial electrical resistance (TEER) of mock-infected, T. gondii-infected, or IL-1β-treated (0.5 ng/ml) HUVEC monolayers is shown. HUVEC were cultured to confluence for 3 days. T. gondii or IL-1β was added at time zero (start), and barrier integrity was measured at 15-min intervals for 18 h by ECIS. Data reflect combined results from three independent experiments and are presented as means ± standard errors of the means (SEM) (error bars). Values that are significantly different by two-way ANOVA with a Bonferroni posttest correction are indicated by asterisks as follows: *, P < 0.05; ***, P < 0.001. (B) Permeability of mock-infected, T. gondii-infected, or IL-1β-treated (0.5 ng/ml) HUVEC monolayers, as measured by transendothelial flux of 40-kDa FITC-dextran at 18 h. Flux is presented as means ± SEM. Data reflect combined results from three independent experiments. Values that are significantly different by one-way ANOVA with a Tukey posttest are indicated by bars and asterisks as follows: *, P < 0.05; ***, P < 0.001.