TABLE 2.
PCR primers used in this study
| Circularization ICE | Sequence (5′→3′) |
Product size (bp) | Reference | |
|---|---|---|---|---|
| Forward primer | Reverse primer | |||
| Tn6685 inverse | GCGTTAGTGGATCGATCGTAG | CACGACGGGTTAAAAACTCA | 508 | This study |
| Tn6686 inverse | CGTAATGTTTGTATCAGCCTTTTT | GTTCTTAAACCGTGGTCAGC | 700 | This study |
| Plasmid gap closure | ||||
| pHblaTEM 1 | TAGCTTCCCGGCAACAATTA | AGCGAAATATCTGGGCTGAA | ca. 400 | This study |
| pHblaTEM 2 | CCCAGATATTTCGCTCTTTCC | TTGTGGGCTGAGTTACAACG | ca. 450 | This study |
| PCRs to confirm transconjugants | ||||
| TEM PCR to detect ICE gene blaTEM-1 | ATGAGTATTCAACATTTCCG | CCAATGCTTAATCAGTGAGG | 858 | 58 |
| Rd217 (Rd-ORF specific PCR) | TCTAATTATCGGCGCGATTT | TCACATCACGATGGAAGGAA | 463 | This study |