Fig 5. In vivo expression of P_cnfY:luxCDABE fusions.

(A) Schematic representation of a transcriptional (PJNS02, [20]) and a translational fusion (pBO6500) of the cnfY promoter region (P_cnfY) to the luxCDABE reporter gene operon. (B) Y. pseudotuberculosis YPIII cultures harboring these plasmids were grown at 25°C and 37°C. Samples for bioluminescence measurement (described in Material and Methods) were taken after 4 hours. The data are depicted as relative light units (RLU/OD₆₀₀) from three independent cultures measured on a CLARIOstar plus reader (BMG Labtech, Ortenberg, Germany). (C) Y. pseudotuberculosis YPIII cultures harboring the translational fusion including the wildtype (pBO65000), the R1 (pBO6501), or the R1+2 (pBO6502) RNAT variant were grown at 25°C and 37°C. Samples for bioluminescence measurement (described in Material and Methods) were taken after 4 hours. The data are depicted as relative light units (RLU/OD₆₀₀) from three independent cultures measured on a Varioskan Flash (Thermo Scientific, St. Leon-Rot, Germany). Results from bioluminescence measurements of variant R1+2 are additionally shown as inlet on a different scale to reflect the low signal intensity.