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. 2020 Jan 17;16(1):e1008184. doi: 10.1371/journal.ppat.1008184

Fig 7. Temperature-controlled ribosome binding to the cnfY RNAT.

Fig 7

(A) Primer extension inhibition of the wild type (pBO4465) and R1+2 cnfY RNAT (pBO4466) in presence (+) or absence (−) of 30S ribosomal subunits was conducted as described in material and methods. The resulting signals correspond to the full-length reverse transcription (RT) product and the termination product (toe print; corresponding to nucleotides +11 to +16 from AUG). ACGT indicate the corresponding DNA sequencing reactions. The SD sequence as well as the ATG codon are labelled. The experiment was performed at least in triplicate (three biological replicates). (B) Toe printing signals (nucleotides +11 to +16 from AUG codon) were quantified by integrated density quantification using AlphaEaseFC software. The mean density ratio (25°C/37°C) and its standard deviation was calculated from three independent experiments.