Figure 4.
Expansion of Gdf5-Cre;tdTom+ epiphyseal bone marrow cells and early attachments at 1 week postsurgery. (A) Tibial tunnel is shown with tendon graft in the center flanked by expanding tdTom+ bone marrow population. Panels A2–5 demonstrate early attachments at the interface of the graft with the marrow space. (A2) toluidine blue O stain, (A3) tdTom channel (red) overlaid onto toluidine blue image, (A4) tdTom channel with DAPI counterstain, and (A5) collagen SHG with tdTom channel. Dotted lines denote interface between dense collagen of graft and looser collagen containing tdTom+ cells (yellow arrow). Scale bars: 200 μm.
