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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Ann N Y Acad Sci. 2019 Oct 9;1460(1):57–67. doi: 10.1111/nyas.14250

Figure 5.

Figure 5.

Gdf5-Cre;tdTom+ cells produce mineralized attachments in the epiphyseal regions of the bone tunnels. (A1) Image of entire knee joint with dotted line denoting tunnel path at 4 weeks postsurgery. (A2) Toluidine blue stained image of tibial tunnel. Panels A3–6 demonstrate zonal attachment at the entrance to the tibial tunnel. (A3) Toluidine blue O image, (A4) tdTom channel (red) with demeclocycline label (yellow), (A5) collagen SHG with demeclocycline, and (A6) tdTom channel with demeclocycline and alkaline phosphatase activity (magenta). Arrows denote yellow demeclocycline tidemark. * denotes mineralized fibrocartilage. Scale bar: 200 μm. (B) quantification of the percentage of tdTom+ cells within alkaline phosphatase regions of the epiphyseal and metaphyseal attachments along with the native PCL entheses. Bars indicate P < 0.017.