Fig. 1.

β-Oxidation up-regulation strategy on intracellular triacylglycerols (TAGs) employed in Ref. [8]. Intracellular TAGs which accumulate in primary metabolism are mobilized to produce polyketides via β-oxidation during stationary phase. Overexpression of the fatty acyl-CoA synthetase (ACS) allows overproduction of acyl-CoA to enter β-oxidation cycle, and therefore increasing the cellular level of acetyl-CoA, the key precursor of polyketides, as well as reducing equivalents and ATP. The high level of the latter two metabolites further inhibit TCA cycles, which in turn weakens the carbon flux from acetyl-CoA to TCA cycle but increases the precursor supply towards polyketide biosynthesis.