TABLE 3.

Volume of collected ascidian embryos for the estimation of the recommended cell number (100,000–180,000 cells) needed for ATAC-seq.

Developmental stage	Volume of embryos (μL)
16 cells	30–50
32 cells	20–25
64 cells	10–12
112 cells	7–10
Late gastrula	7–10
Mid neurula	7–10