Table 2.

Summary with details about gradient methods used in the study

Experiment	Ion pair (mM)	Column	Gradient start (v% MeCN)	Gradient slope (v% mL−1)
See Figs. 1, 2, and 3	50 mM TEtAA	C4, C8, C18, and phenyl	10.15	0.47
	5 mM TBuAA		36	0.95
See Fig. 4	10 mM TBuAA	C8	45.6	1.14
	20 mM TBuAA		48.6
	30 mM TBuAA		49.8
See Figs. 5, 6, and 7	30 mM TBuAA	C4	51.6	1.14
		C8	51.6
		C18	51.3
		Phenyl	49.2
See Fig. 6 (purity analysis)	10 mM TBuAA	BEH C18	30	1.6