Table 2.
Protein purification methods attempted in this study
| Purification method | Protocol modificationsc | Source |
|---|---|---|
| Ethanol | 9 volumes of ethanol | [46] |
| 100% acetone | – | [47] |
| 80% acetone | – | [48] |
| TCA/acetone | 13.3% TCA | [45] |
| Optimized TCA/acetone | Glass beads | [10] |
| Phenol:methanol:ammonium acetate | – | [49] |
| Chloroform:methanol | – | [50] |
| PCIa | Collect organic phase | [51] |
| Methanol:MTBE:water | – | [35] |
| Butanol/di-isopropyl ether | Collect aqueous phase | [52] |
| Sequentialb | – | [9] |
| PEG 6000 | – | [27] |
| Hexane:ethanol | Ethanol replaced isopropanol, 60 °C incubation for 30 min | [38] |
| Size exclusion | Modified PES 10 kDa, 500 μL | VWR, Germany |
| Ammonium sulfate | – | [53] |
| DISSOLVAN® 7:ethanol | Adapted protocol from (38) | Clariant |
| DISSOLVAN® 5:ethanol | Adapted protocol from (38) | Clariant |
aPCI phenol/chloroform:isoamyl alcohol
bSequential (TCA/acetone + phenol)
cModifications made to adopted protocols