Figure 1.

Comparison of CENH3 protein sequences identified in C. europaea (CEURO), C. campestris (CCAMP), and C. japonica (CJAPO). The alignment of CENH3 protein sequences is shaded to highlight invariable sites (black) and sites containing similar amino acid residues (gray). These sites are also marked below the alignment by asterisks and dots, respectively. Note that the sequences are relatively conserved at their C-termini, which contain the histone fold domain (HFD), but are highly divergent at the N-terminus. Sequences of peptides used to produce CENH3-specific antibodies are underlined.