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. 2020 Jan 30;11:598. doi: 10.1038/s41467-020-14382-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Strains were grown as described under antimicrobial susceptibility testing in the Methods section and spread onto either Brucella Blood Agar (BBA) plates, or onto BHIY/CDSS agar plates (BHI). E-tests were placed, and plates were incubated for 48 h before imaging. In all, 2 mg L⁻¹ is the EUCAST epidemiological cutoff for metronidazole that was used to define resistance in this study³². E-test values for the indicated strains are shown next to their respective panels. The images represent three independent repeats with a single replicate per condition. Source data are provided as a Source Data file.