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. 2020 Jan 30;10:1522. doi: 10.1038/s41598-020-58470-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structure of gut on different stages of regeneration in holothurian Eupentacta fraudatrix. (A) Connective-tissue thickening on the edge of mesentery on day 3 post-evisceration (first stage). (B) Gut anlage on day 6 post-evisceration (second stage); arrow indicates site of migration of coelomic epithelial cells into connective-tissue thickening, asterisks show clusters inside the intestinal anlage. (C) Digestive tube on day 12 post-evisceration (third stage). bw – body wall, g – digestive tube, ga – gut anlage, gd – gonoduct, il – intestinal lining, m – mesentery.