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. 2020 Jan 30;11(1):77. doi: 10.1038/s41419-020-2280-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The timeline for the entire study, including sample collection time points and the experiments that were performed at different time points. b Aif1 mRNA expression was upregulated in AIF Tg mice compared to WT mice at P9 under physiological conditions (5.89 ± 0.80 vs. 1.00 ± 0.08, n = 6/group, ***p < 0.001). c Aif2 mRNA expression was not significantly different between WT and AIF Tg mice at P9 (1.00 ± 0.11 vs. 0.93 ± 0.09, n = 6/group, p = 0.227). d Representative immunoblots of the mitochondrial fraction from cortical tissue of P9 WT and AIF Tg mice. A greater amount of exogenous AIF (upper band of AIF) than endogenous AIF (lower band of AIF) was seen in the AIF Tg mice. e Total AIF protein expression in AIF Tg mice (two bands) was dramatically increased compared to WT mice (one band) (2.44 ± 0.13 vs. 1.00 ± 0.17, respectively, n = 6/group, ***p < 0.001), but there was no significant difference in endogenous AIF expression (1.08 ± 0.14 vs. 1.00 ± 0.31, respectively, n = 6/group, p = 0.5577). f Semi-quantification of some mitochondria-related proteins (CHCHD4, COX1, CYTC, and SOD2) did not show any significant differences between WT and AIF Tg mice under physiological conditions (n = 6/group). g OXPHOS complex I activity was determined in the mitochondrial fraction of cortical tissue of P9 WT and AIF Tg mice, and no significant difference was found (13.04 ± 3.32 vs. 14.05 ± 2.58, n = 6/group, p = 0.5677). h The body weight of P9 mouse pups was not significantly different between WT and AIF Tg mice (5.06 ± 0.82 g vs. 5.28 ± 0.74 g, respectively, n = 54/group, p = 0.1407). i The body weight at one year of age was not significantly different between WT and AIF Tg mice (40.18 ± 8.04 g vs. 43.61 ± 9.13 g, respectively, n = 8 in WT mice, n = 13 in AIF Tg mice, p = 0.393). j The DCX-positive cell density in the granular layer of the dentate gyrus of AIF Tg mice was significantly higher than WT mice at one year of age (3.93 ± 0.66 cells/mm vs. 5.15 ± 0.71 cells/mm, n = 8 in WT mice, n = 6 in AIF Tg mice, **p < 0.01). k Representative pictures of DCX staining in dentate gyrus regions in WT and AIF Tg mice at one year of age. Black arrows indicate the positive cells.