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. 2020 Jan 30;11(1):77. doi: 10.1038/s41419-020-2280-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative active caspase-3 staining in the cortex and striatum regions in WT and AIF Tg mice at 24 h post-HI. b Higher magnification of active caspase-3 staining images showing the active caspase-3-positive cells in the cortex (Cx) (upper panels) and hippocampal CA1 area (lower panels) in WT and AIF Tg mice at 24 h post-HI. c Quantification of active caspase-3-positive cells in WT and AIF Tg mice at 24 h after HI in the cortex (156.1 ± 50.6 cells/mm² vs. 254.3 ± 106.7 cells/mm², 95% CI 1.0–195.4, respectively, n = 7/group, *p < 0.05) and CA1 (1758.3 ± 219.5 cells/mm² vs. 1,739.8 ± 252.7 cells/mm², 95% CI −272.3–235.3, respectively, n = 8/group) area. d Higher magnification of active caspase-3 staining showing the active caspase-3-positive cells in the striatum (Str) (upper panels) and habenular nuclei (HN) (lower panels) in WT and AIF Tg mice at 24 h post-HI. e Quantification of active caspase-3-positive cells in WT and AIF Tg mice at 24 h after HI in the striatum (770.4 ± 85.5 cells/mm² vs. 938.1 ± 80.3 cells/mm², 95% CI 78.8–256.7, respectively, n = 8/group, **p < 0.01) and habenular nuclei (205.7 ± 69.6 cells/mm² vs. 235.5 ± 97.9 cells/mm², 95% CI −61.4–120.9, respectively, n = 8/group). f The caspase-3 activity in the cortical tissue was measured at 24 h after HI. The activity was increased dramatically in the IL hemisphere, but there was no significant difference between WT and AIF Tg mice (n = 6/group). g Immunoblotting of PARP-1 and cleaved PARP-1 in the nuclear fraction from the cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. Lamin B was used as the loading control. h Quantification of PARP-1 and cleaved PARP-1 did not show any significant difference between WT and AIF Tg mice after correction for the loading control (n = 5/group).