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. 2020 Jan 30;11:589. doi: 10.1038/s41467-020-14470-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a and b Cellular localization of mCherry-PER1 a and the statistical analysis of nuclear-localized mCherry-PER1 b in the liver from mice fasted from ZT12 to ZT4 or refed for another 2 h from ZT4 to ZT6. c Immunoblots showing amounts of PER1 in nuclear fractions of liver extracts from mice fasted from ZT12 to ZT4 or refed for another 2 h from ZT4 to ZT6. d–f Statistical analysis of PER1 d and CRY1 e in Nucl. and Rev-Erbα in TCL f from immunoblots as shown in c. g qPCR results showing the relative mRNA level of Rev-Erbα in liver extracts from mice fasted from ZT12 to ZT4 or refed for another 2 h from ZT4 to ZT6. Data are shown as mean ± s.e.m. Comparison of different groups was carried out using two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, n = 5–7 mice. Source data are provided as a Source Data file.