Fig. 5. Nano-sapper exhibited self-enhanced accumulation in PDAC.
a Six-week-old male C57BL/6 mice were orthotopically inoculated with KPC1199 cells (1 × 106) on day 0. Saline, FHK-CaMP (MP = 13.9 mg/kg), FHK-pLIGHT@CaP (25 μg plasmid per mouse) and Nano-sapper (MP = 13.9 mg/kg, 25 μg plasmid per mouse) were i.v. administered on day 11, 13, 15, and 17, and DiR-labeled CaP was i.v. administered on day 11, 14, and 18 (DiR = 1 mg/kg), and subjected to IVISTM for in vivo imaging. On day 18, tumors were harvested for ex vivo detection. b, c DiR signal was monitored to reflect the accumulation of CaP. H, heart; Li, liver; S, spleen; Lu, lung; K, kidney (n = 3 mice). d Tumors were extracted to detect DiR signal (n = 3 mice). Data are presented as mean ± s.d. One-way ANOVA with Bonferroni multiple comparisons post-test was used for (c) and (d). Error bars represent s.d. Source data of (c) are provided as a Source Data file.