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. 2020 Jan 30;11:622. doi: 10.1038/s41467-020-14425-7

Fig. 6. Nano-sapper enhanced CTLs infiltration and initiated the TLSs formation in TME.

Fig. 6

a, b Tumor slices were stained for MECA79 and analyzed by ImageJ. Scale bars, 100 μm. n = 10 section images from three mice. The experiments were repeated three times independently. c, d Flow cytometry analysis of immune cells within TME (n = 3 mice). e, f Nano-sapper treatment promoted T cell penetration into deeper region of PDAC and induced the formation of TLSs. Scale bars, 100 μm. Data were pooled from three independent experiments. g, h Chemoattractants involved in lymphocyte recruitment were elevated within TME. (For CCL21, Saline, n = 4 mice; FHK-CaMP, n = 11 mice; FHK-pLIGHT@CaP, n = 11 mice; Nano-sapper, n = 10 mice. For CXCL13, Saline, n = 6 mice; FHK-CaMP, n = 6 mice; FHK-pLIGHT@CaP, n = 12 mice; Nano-sapper, n = 12 mice.) Data are presented as mean ± s.d. One-way ANOVA with Bonferroni multiple comparisons post-test was used for (b), (c), (d), (g), and (h). Error bars represent s.d.