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. 2020 Jan 30;10:1477. doi: 10.1038/s41598-020-58406-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HGQ stabilization by G-quadruplex on gene expression evaluated through mTFP-based reporter assay. (a) Schematic representation of the cloning strategy. The HGQ of the NiV gene and its mutant sequence were cloned just after the ATG start codon of the mTFP gene. Under normal condition the mTFP gene would be translated to produce TFP protein, but in presence of stabilized G-quadruplex, the translation process will be hindered leading to attenuation of the TFP expression. (b) TMPyP4 and Braco-19 suppress the mTFP expression in the HEK cells as compared to the untreated or TMPyP2 treated cells due to stabilization of the HGQ structure. (c) No clear suppression in mTFP expression was observed for HGQ mutant sequence upon treatment with TMPyP4 or Braco-19. Scale-50 µm.