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. 2020 Jan 30;11(1):78. doi: 10.1038/s41419-020-2281-y

Fig. 5. Caspase-1 knockout suppresses AT1-AA production in PE mice.

Fig. 5

a Effect of caspase-1 knockout on AT1-AA expressions in PE mice. b Effect of caspase-1 knockout on spleen size in PE mice. c Effect of caspase-1 knockout on spleen pathological features in PE mice. Representative H&E staining images of spleen are showed. The bar is 250 μm. d, e IHC analysis of spleen and lymph node. Sections of spleen (d) and lymph node (e) were prepared from control, PE and Casp1−/− mice, and stained with antibody against CD68 (red) and TUNEL (green); staining profiles were merged in the third column. Bar = 100 μm. f, g IHC analysis of spleen and lymph node. Sections of spleen (f) and lymph node (g) were prepared from control, PE and Casp1−/− mice, and stained with antibody against IgG (red). Bar = 200 μm. h Effect of caspase-1 knockout on peripheral AT1-AA-producing CD19+CD5+ B cells in PE mice. CD19+CD5+ B cells in peripheral blood of mice were stained with APC-conjugated Rat Anti-Mouse CD19 and PE-conjugated Rat Anti-Mouse CD5, and subjected to flow cytometry analysis. Results are expressed as means ± SEM (n = 7 mice in each group). **P < 0.01 and ***P < 0.001 versus control group, †P < 0.05, ††P < 0.01 and †††P < 0.001 versus LPS group, one-way ANOVA with S-N-K posttest.