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. 2020 Jan 30;11:612. doi: 10.1038/s41467-020-14511-w

Fig. 6. H3R2me2a is involved in CPC translocation and cytokinesis.

Fig. 6

a The images are maximum projections from z stacks of representative cells that were stained for H3R2me2a (red) and DNA (blue). The arrow and the arrowhead point to metaphase and anaphase chromosomes, respectively. b–d The HeLa cells were treated with siRNA or an inhibitor against PRMT6, and the levels of CPC components during anaphase from three independent experiments were quantified (n = 30 anaphase cells). CPC (green), β-tubulin (red), and DNA (blue). e The fraction of bi-nucleated or multinucleated cells in HeLa cells depleted of PRMT6 was counted and plotted (n = 300 interphase cells from three independent experiments). f The fraction of binucleated or multinucleated cells in HeLa cells transfected with Flag-histone H3 WT or mutants was counted and plotted (n = 300 interphase cells from three independent experiments). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01).