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. 2020 Jan 30;11:620. doi: 10.1038/s41467-020-14484-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Differential interference contrast (DIC) image of Sf9 cells 6 days p. i. with recombinant baculovirus containing the gene for TbIMPDH. Needle-shaped crystals, mostly extending the cell-body, are clearly visible. The arrowhead points to a vertical crystal, indicating the rectangular shape of the needles. b Transmission electron micrograph of a cross section of two TbIMPDH crystals within the cytoplasm of a Sf9 cell 6 days p. i. showing a clear crystal lattice with large water channels. C TbIMPDH crystal, CP cytoplasm, ER endoplasmic reticulum, N nucleus, NM nuclear membrane, P10 baculoviral P10 protein aggregate, PM plasma membrane, VP - viral particles. c Modulation contrast image of Sf9 cells showing multiple TbIMPDH crystals 7 days p. i., located within living cells and free in the surrounding medium (arrowhead). d Growth of a TbIMPDH crystal at 5 days p. i. in Sf9 cells taking place over the course of several hours. The crystal grows simultaneously in two dimensions. See also Supplementary Movie 1.