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M5BT-specific IgG and IgG subtype immune responses at 4 weeks postimmunization. The anti-M5BT serum immunoglobulin titers were measured using blocking ELISA. Note that the endpoint titers of the result are represented on a log scale on the y-axis. A Anti-M5BT total IgG titers, B IgG1 titers and C IgG2a titers. All values represent the mean ± SD (n = 5). Significant differences were compared to the untreated group. *: p < 0.05; **: p < 0.01; ***: p < 0.001, one-way ANOVA
