Table 2.
Immunization groups and vaccine dose used in the in vivo immunization study
| Groups | No. mice | Vaccine dose/total volume | Total no. injection | Adjuvant | Annotation |
|---|---|---|---|---|---|
| Untreated | 5 | – | 3 | – | Untreated |
| iFMDV | 5 | 80 µl | 3 | – | iFMDV |
| M5BT | 5 | 10 µg/80 µl | 3 | – | M5BT |
| M5BT (CFA) | 5 | 10 µg/80 µl | 3 | CFA/IFA | CFA |
| M5BT/IA MP | 5 | 10 µg/80 µl | 3 | – | IA |
| M5BT/M-IA MPs | 5 | 10 µg/80 µl | 3 | M-IA |
iFMDV, inactivated foot-and-mouth disease vaccine; M5BT, a multi-epitope recombinant protein which consists of tandem repeats of five B-cell epitopes derived from the GH loop of different FMDV variants and one T-cell epitope; CFA, Complete Freund’s Adjuvant; IA, inulin acetate; M-IA, mannan-decorated inulin acetate