TABLE 1.

Isolation conditions, library preparation information, genome sequencing statistics, and accession numbers for the described acidobacterial genomes

Strain	Isolation source; yr	Isolation and growth conditions (reference)	Library prepn	No. of reads (technology)	Avg read length (bp)	Assembler (reference)	No. of contigs (no. of scaffolds)	N/L50 of:		Avg genome coverage (×)	Genome size (Mb)	G+C content (mol%)	Assembly level	GenBank accession no.	SRA accession no.
								Scaffolds	Contigs
KBS 63	Kellogg Biological Station, Hickory Corners, MI, USA, grassland soil (treatment 8); 2003	VSB-7 mix organic C substrates in CO2-enriched air (5)	454 paired-end library;a Illumina std. shotgun libraryb	69,481,460 (Illumina Solexa); 514,324 (454 paired end)	76 (Illumina Solexa); 151 (454 paired end)	Newbler v2.3-Prerelease 6/30/2009, Velvet v1.0.13 (13)	33 (2)	1/5.2 Mb	1/5.2 Mb	1083.1 (Illumina Solexa); 8.5 (454 paired end)	5.23	60.3	Complete	CP003379	SRS1568612
KBS 83	Kellogg Biological Station, Hickory Corners, MI, USA, agricultural soil (treatment 1); 2006	VL55 mix of plant polymeric C in air (7)	Illumina std. shotgun libraryb	12,985,884 (Illumina HiSeq 2000)	2 × 150	Velvet v1.1.04 (13), wgsime , Allpaths-LG v r41043 (14)	25 (25)	5/504.7 kb	5/504.7 kb	390	6.25	59.2	Permanent draft	ARMD00000000	SRS844143
KBS 89	Kellogg Biological Station, Hickory Corners, MI, USA, grassland soil (treatment 8); 2002	VSB-6.8 mix organic C substrates, acyl homoserine lactones in CO2-enriched air (4, 5)	Illumina std. shotgun libraryb	12,475,762 (Illumina HiSeq 2000)	2 × 150	Velvet v1.1.04 (13), wgsime , Allpaths-LG v r41043 (14)	14 (14)	3/943.6 kb	3/943.6 kb	122.6	6.01	57.6	Permanent draft	ARME00000000	SRS844144
KBS 146	Kellogg Biological Station, Hickory Corners, MI, USA, grassland soil (treatment 8); 2006	VL55 mix of organic C substrates, CO2-enriched hypoxia (11)	PacBio SMRTbell libraryd	185,131 (PacBio RS platform)	3,493 ± 2,894	HGAP v2.0.0 (15)	2 (2)	1/5.0 Mb	1/5.0 Mb	64.1	5.00	56.7	Permanent draft	JHVA00000000	SRS1534005
TAA 43	Hindgut of Reticulitermes flavipes (Kollar) (Phinotermitidae), Dansville, MI, USA; 2002	VSB-7 yeast extract and peptone in CO2-enriched air (4, 5)	Illumina std. shotgun libraryb	13,649,630 (Illumina std. paired end, Illumina HiSeq 2000)	2 × 151	Velvet v1.2.07 (13), wgsime , Allpaths-LG v r46652 (14)	7 (7)	1/3.5 Mb	1/3.5 Mb	302.0	4.95	56.7	Permanent draft	JUGR00000000	SRS1366045
TAA 166	Hindgut of R. flavipes (Kollar) (Phinotermitidae), Dansville, MI, USA; 2002	VSB-7 yeast extract and peptone in CO2-enriched air (4, 5)	Illumina std. shotgun libraryb and long-insert mate pair libraryc ; PacBio SMRTbell libraryd	40,920,398 (Illumina CLIP paired end, Illumina HiSeq 2000); 15,055,388 (Illumina std. paired end, Illumina HiSeq 2000); 233,258 (PacBio RS platform)	2 × 90; 2 × 150; 1 × 2259	AllpathsLG vr42328 (14)	3 (3)	1/4.7 Mb	1/4.7 Mb	973.9 (Illumina); 85.4 (PacBio)	6.14	58.8	Permanent draft	ATWD00000000	SRS438888
KBS 96	Kellogg Biological Station, Hickory Corners, MI, USA, agricultural soil (treatment 1); 2006	VL55 mix of plant polymeric C in air (7)	Illumina std. shotgun libraryb and long-insert mate pair libraryc	14,485,381 (Illumina std. paired end, HiSeq 2000); 58,296,769 (Illumina CLIP paired end, Illumina HiSeq 2000)	2 × 150; 2 × 89	Allpaths-LG v r41043 (14)	13 (2)	1/6.7 Mb	2/1.6 Mb	1,098.6	6.69	57.2	Permanent draft	ARMF00000000	SRS438892
MPL3	Acidic Sphagnum peat bog, Bakchar, Tomsk region, West Siberia; 2004	Biofilm-mediated enrichment approach (6, 12)	PacBio SMRTbell libraryd	208,346 (PacBio RS platform)	3,087 ± 2,325	HGAP v2.0.0 (15)	4 (4)	1/4.3 Mb	1/4.3 Mb	176.8	5.75	57.0	Permanent draft	JNIF00000000	SRS1520682

^a454 Titanium, paired ends, 8 kb; 15 μg genomic DNA are sheared by the Hydroshear to ~8-kb size fragments. The sheared samples are then gel selected for the 8-kb bands, purified, and ligated to the 42-bp loxP linkers on either end. These loxP linkers are labeled by biotin. The loxP linker-ligated fragments are then circularized by the Cre recombinase. As a result, the ends of 20-kb fragments are brought together and bridged by a single loxP linker. These circular DNAs are further sheared to 500-bp fragments, and the fragments carrying the loxP linkers are recovered by the Streptavidin-coated magnetic beads. Consequently, the loxP linker-containing fragments are ligated to the 454 Titanium adapters A and B in the same way that the shotgun libraries are created. The 454 library fragments are then clonally amplified in bulk by capturing them through hybridization on microparticle beads and subjecting them to emulsion-based PCR. This results in beads that are covered with millions of copies of a single DNA fragment (range, 400--800 bp), where each bead contains a different clonally amplified library fragment. After amplification, the beads are recovered from the emulsions and are loaded into the wells of a PicoTiterPlate (PTP) device such that wells contain single DNA beads. The PTP device is then inserted into the 454 genome sequencer FLX-Titanium instrument for sequencing where sequencing reagents are sequentially flowed over the PTP wells. Each incorporation of a nucleotide complementary to the template strand results in a chemiluminescent light signal that is recorded by a camera, and the sequence of the DNA fragments is determined. This sequencing-by-synthesis method is known as pyrosequencing.

^bIllumina regular fragment, 300 bp; 100 ng of DNA was sheared to 300 bp using the Covaris LE220 instrument and size selected using solid-phase reversible immobilization (SPRI) beads (Beckman Coulter). The fragments were treated with end repair, A-tailing, and ligation of Illumina-compatible adapters (IDT, Inc.) using the KAPA-Illumina library creation kit (Kapa Biosystems). The prepared libraries were quantified using Kapa Biosystem’s next-generation sequencing library quantitative PCR (qPCR) kit and run on a Roche LightCycler 480 real-time PCR instrument. The quantified libraries were then multiplexed with other libraries, and the pool of libraries was then prepared for sequencing on the Illumina HiSeq or the Illumina GAIIx (KBS 63) sequencing platform utilizing a TruSeq paired-end cluster kit v3 or a paired-end cluster generation kit, v4 (KBS 63) and Illumina’s cBot instrument to generate a clustered flow cell for sequencing. Sequencing of the flow cell was performed on the Illumina HiSeq 2000 sequencer using a TruSeq SBS sequencing kit v3 following a 2 × 150 indexed run recipe and on the Illumina GAIIx sequencer using SBS sequencing kits, v4, following a 2 × 76 run recipe (KBS 63).

^cIllumina regular long mate pair, 8 kb, Cre-Lox random shear mate pair sequences; 5 μg of DNA was sheared using = Covaris g-TUBEs and was gel size selected for 8 kb. The sheared DNA was treated with end repair and ligated with biotinylated adapters containing loxP. The adapter-ligated DNA fragments were circularized via recombination with a Cre excision reaction (NEB). The circularized DNA templates were then randomly sheared using the Covaris LE220 instrument. The sheared fragments were treated with end repair and A-tailing using the KAPA-Illumina library creation kit (Kapa Biosystems) followed by immobilization of mate pair fragments on streptavidin beads (Invitrogen). Illumina-compatible adapters (IDT, Inc.) were ligated to the mate pair fragments, and 14 cycles of PCR were used to enrich for the final library (Kapa Biosystems). The prepared libraries were quantified using Kapa Biosystem’s next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument. The quantified libraries were then multiplexed with other libraries, and the pool of libraries was then prepared for sequencing on the Illumina HiSeq sequencing platform utilizing a TruSeq paired-end cluster kit v3 and Illumina’s cBot instrument to generate a clustered flow cell for sequencing. Sequencing of the flow cell was performed on the Illumina HiSeq 2000 sequencer using a TruSeq SBS sequencing kit v3 following a 2 × 100 indexed run recipe.

^dPacBio >10-kb libraries with AMPure bead size selection; unamplified libraries were generated using the Pacific Biosciences standard template preparation protocol for creating >10-kb libraries. A total of 5 μg of genomic DNA was used to generate each library, and the DNA was sheared using Covaris g-TUBEs to generate sheared fragments >10 kb in length. The sheared DNA fragments were then prepared using Pacific Biosciences SMRTbell template preparation kit v1.0, where the fragments were treated with DNA damage repair, had their ends repaired so that they were blunt ended, and were 5′ phosphorylated. Pacific Biosciences hairpin adapters were then ligated to the fragments to create the SMRTbell template for sequencing. The SMRTbell templates were then purified using exonuclease treatments and size selected using AMPure PB beads. Sequencing primer was then annealed to the SMRTbell templates, and version XL sequencing polymerase was bound to them. The prepared SMRTbell template libraries were then sequenced on a Pacific Biosciences RS II sequencer using version C2 chemistry and 2-h sequencing movie run times.

^ehttps://github.com/lh3/wgsim.