Skip to main content
. 2020 Jan 23;52:102632. doi: 10.1016/j.ebiom.2020.102632

Fig. 6.

Fig 6

In vivo anti-tumour effect of DLL3-targeted NIR-PIT.

(a) DLL3-targeted NIR-PIT regimen involving rova-IR700 injection and NIR light exposure is shown in a line.

(b) In vivo fluorescence and the BLI of tumour-bearing mice. In the NIR-PIT group, fluorescence and luminescence was decreased after irradiation with NIR light. Scale bars, 20 mm.

(c) Tumour volume (mm3) is shown in the left graph while the ratio is shown in the right (before NIR-PIT = 1). Prior to NIR-PIT, tumours were almost the same size. NIR-PIT introduced on days 0 and 1 led to significant reductions in the tumour volume ratio whereas neither rova-IR700 injection nor NIR light irradiation alone affected tumour size (n ≥ 7 in each group.) (PIT group vs. control group at day 3: *p = 0.0212; PIT group vs. control group at day 7: *p = 0.0084; PIT group vs. light only group at day 7: *p = 0.0123; PIT group vs. light only group at day 10: *p = 0.0136; light only group vs. control group at day 3, 7, 10: p> 0.9999; i.v. only group vs. control group at day 3, 7, 10: p > 0.9999, Kruskal–Wallis test with Dunn's post-test).

(d) Quantitative RLU showed a significant decrease in DLL3-targeted NIR-PIT-treated tumours (n = 5 in each group) (PIT group vs. light-only group in (b): *p = 0.0007; PIT group vs. control group at (c): *p = 0.0385, Kruskal–Wallis test with Dunn's post-test).

(e) DLL3-targeted NIR-PIT led to prolonged survival in tumour-bearing mice (n ≥ 7 in each group) (*p = 0.023, log-rank test).

(f) DLL3-targeted NIR-PIT regimen of SBC5-DLL3-luc-GFP xenografts in the dual-dorsal tumour mouse model.

(g) In vivo fluorescence and BLI of xenografts in the dual-dorsal tumour model. One day after rova-IR700 administration, only the tumour on the right was irradiated with NIR light. Fluorescence and luminescence were diminished only in the right tumour but not in the left. The site-specific effect in vivo was successfully performed. BLI, bioluminescence imaging; GFP, green fluorescent protein; NIR, near infrared; PIT, photoimmunotherapy; RLU, relative light unit. Scale bars, 20 mm.