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. 2020 Jan 23;52:102633. doi: 10.1016/j.ebiom.2020.102633

Fig. 2.

Fig 2

Dysfunctional angiogenesis of FD-VECs. (a) Abnormal tube-like structure of FD-VECs. Unlike WT-VECs, FD-VECs do not form tubular structures regardless of enzyme treatment. Scale bar, 200 μm. (b) Transcriptional expression of angiogenic and anti-angiogenic factors in WT- and FD-VECs. The data are expressed as means ± SEM (n = 3). * p < 0.05; ** p < 0.01. (c) Expression of angiogenic and anti-angiogenic factors in FD-VECs. The angiogenic factors VEGF and ANG2 are down-regulated, while TSP-1 is up-regulated in FD-VECs compared to WT-VECs. (d) Immunostaining of TSP-1 in FD-VECs. TSP-1 is overexpressed in FD-VECs regardless of enzyme treatment when compared with WT-VECs. Scale bar, 50 μm. (e) Reduced expression of KDR and eNOS in FD-VECs. The relative protein ratios were quantified using the ImageJ software. The data are expressed as means ± SEM (n = 3). * p < 0.05; ** p < 0.01; *** p < 0.001.