Skip to main content
. 2020 Jan 23;52:102633. doi: 10.1016/j.ebiom.2020.102633

Fig. 6.

Fig 6

Correction of the GLA mutation in FD-1-iPSCs using the CRISPR/CAS9 system. (a) GLA activity in FD-1-Cor-iPSCs. The data are expressed as means ± SEM (n = 3). *** p < 0.001. (b) GLA activity in FD-1-VECs. The data are expressed as means ± SEM (n = 3). *** p < 0.001. (c) Angiogenesis recovery in FD-1-Cor-VECs. Like WT-VECs, FD-1-Cor-VECs form tube-like structures. Scale bar, 200 μm. (d) Rescue of SMAD2 signaling in FD-1-Cor-VECs. Compared to their levels in FD-1-VECs, correction of the mutated GLA gene increases the expression of KDR and eNOS and reduces the levels of TSP-1 and p-SMAD2. The data are expressed as means ± SEM (n = 2). ** p < 0.01. (e) Immunostaining of TSP-1 in FD-1-Cor-VECs. Expression of TSP-1 is reduced in FD-1-Cor-VECs to a level similar to that of WT-VECs. Scale bar, 50 μm.