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. 2020 Jan 23;6(1):e03175. doi: 10.1016/j.heliyon.2020.e03175

Figure 3.

Figure 3

Cx43 protein expression in LMNA mutant cardiomyocytes. (A) Western blot showing Cx43 expression (left) and the results from western blots (n = 4) indicating no significant difference between the groups (right), p = 0.5038. (B) Cx43 localization in control (NT) cardiomyocytes. Fluorescence images of Connexin 43 (red dots), sarcomeric α-actinin (gray), LMNA GFP (green) and Hoechst (blue) staining of NRVMs (C) Cx43 localization in (from left to right): wild-type LMNA, LMNAE161K, LMNAD192G, LMNAN195K. Upper panels: Sarcomeric alpha-actinin and connexin 43. Lower panels LMNA GFP expression and connexin 43. (D) Quantification of Cx43 gap junction area: significant differences on Cx43 gap-junction were observed between LMNAE161K, LMNAD192G and LMNAN195K and the non-transfected NT cells and WT. Data are presented as mean ± S.D. (n = 5). 161 vs non-treatment: ***p value: <0.0005; 192 vs non treatment: **p value: <0.0045; 195 vs non treatment: ***p value: <0.0001; 161 vs WT: ***p value: <0.0009; 192 vs WT: **p value: <0.0086; 195 vs WT: ***p value: <0.0002. Non-significant differences were observed between the mutant cells and between WT and NT. A full, non-adjusted image is provided in supplementary material (Figure S1).