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. 2020 Jan 24;10:1683. doi: 10.3389/fpls.2019.01683

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Copyright © 2020 Song, Luo, Wang, Fan, Wang, Yang and Jia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of flavonol concentration and relative transcript levels of majority genes encoding flavonoid biosynthetic enzymes in WT and 35S:NtMYB12 plants under HP and LP conditions. (A–B) QueRut and KaeRut concentrations of WT and overexpression tobacco plants. (C–F) Effect of NtMYB12 overexpression on relative transcript levels of flavonol biosynthetic genes in tobacco. Sixteen-day-old seedlings were transferred to the full-strength culture solution and supplied with high Pi (HP; 1mM Pi) or low Pi (LP; 0.02mM Pi) for 21 days. The whole plants of the WT and transgenic plants were used for RNA extraction. FW, fresh weight; CHS, chalcone synthase; CHI, chalcone isomerase; F3H, flavanone 3-hydroxylase; FLS, flavonol synthase. Data are the means ± SDs of five biological replicates. Different letters indicate significant differences (P < 0.05).