Table 1.

CpG and UpA dinucleotide composition in ZIKV variants.

Genomic region	ZIKV variants	Substitutionsa	Number of CpGs	Relative overrepresentation of CpG dinucleotidesb	O/E CpG ratioc	Number of UpAs	Relative overrepresentation of UpA dinucleotidesb	O/E UpA ratioc
E	Wild-type	–	35	1.00	0.38	43	1.00	0.46
	Permuted	195	35	1.00	0.38	43	1.00	0.46
	E+32CpG	135	67	1.91	0.73	42	0.98	0.45
	E+102CpG	280	137	3.91	1.48	43	1.00	0.46
	E/NS1+176CpG	280	137	3.91	1.48	43	1.00	0.46
NS1	Wild-type	–	25	1.00	0.40	34	1.00	0.53
	Permuted	0	25	1.00	0.40	34	1.00	0.53
	E+32CpG	0	25	1.00	0.40	34	1.00	0.53
	E+102CpG	0	25	1.00	0.40	34	1.00	0.53
	E/NS1+176CpG	176	99	3.96	1.51	28	0.82	0.45
ORFd	Wild-type	-	316	1.00	0.45	337	1.00	0.53
	Permuted	195	316	1.00	0.45	337	1.00	0.53
	E+32CpG	135	348	1.10	0.50	336	1.00	0.53
	E+102CpG	280	418	1.32	0.60	337	1.00	0.53
	E/NS1+176CpG	456	492	1.56	0.70	331	0.98	0.53

^aNumber of nucleotide changes vs. the WT ZIKV variant.

^bRelative overrepresentation vs. the WT ZIKV variant.

^cRatio of observed dinucleotide frequency to estimated (O/E ratio).

^dORF, open reading frame.