Table 3.

In vitro competition assay with ZIKV variant-specific restriction digestion.

		Competing mutant # 2
		E+102CpG	E+32CpG	Permuted	Wild-type
Competing mutant #1a	E/NS1+ 176CpG	–b – –	– – –	– – –	– – –
	E+102CpG		– – –	– – –	– – –
	E+32CpG			– ≈ ≈	– ≈ ≈
	Permuted				≈ ≈ +

^aRelative fitness of the competing mutant #1 over the competing mutant #2 was evaluated in triplicate based on the digestion pattern (Supplementary Figure 1).

^b“–”: no competing mutant #1 after passaging; “+”: no competing mutant #2 after passaging; “≈”: both mutants present after passaging.