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Immunostaining of islet markers on day 19. (A) Immunostaining of islet cell markers Glucagon and Insulin on day 19. Cells in M 1 forming a cell aggregate like islet-like cells in the differentiated culture were colocalized with Insulin and Glucagon expression, and cells in M 1 at the edge of the cell aggregate were Glucagon positive. (B) Immunostaining of islet cell markers Somatostatin and Insulin on day 19 in M 1. Scale bars: 100 μm. Undifferentiated DPSCs were used as controls.
