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. 2020 Jan 24;10:1576. doi: 10.3389/fphar.2019.01576

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Copyright © 2020 Xu, Fan, Zhao, Li, Wang, Wang, Wang, Guan and Niu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentiation of hDPSCs into IPCs in Matrigel. (A) Analysis of the growth morphology of hDPSCs in Matrigel. a. Matrigel physical embedded cells before induction; b. Growth status of cells in Matrigel after induction; c-d. HE staining analysis of different cell aggregates after induction. Scale bars: 20 μm. (B) Gene expression analysis Pdx1, Nkx6.1, Insulin and Glucagon by qPCR. Cells in 2D group were used as control. Results are the average of three independent experiments. *p < 0.05. (C) Immunostaining of pancreatic cell markers Pdx1 and Insulin on day 19. Scale bars: 20 μm.