Table 4.

Cytotoxicity Data of Withanolides from Aeroponically Cultivated Physalis peruviana against a Panel of Selected Tumor Cell Lines and Normal Cells^a

	cell lineb
compound	LNCaP	22Rv1	ACHN	M14	SK-MEL-28	HFF
6	>2	0.89 ± 0.10	>2	>2	>2	>2
8	0.33 ± 0.04	0.56 ± 0.01	>2	>2	>2	>2
9	0.94 ± 0.10	0.99 ± 0.08	>2	>2	>2	>2
11	0.29 ± 0.05	0.21 ± 0.02	>2	>2	>2	>2
12	0.16 ± 0.04	0.09 ± 0.01	>2	>2	>2	>2
13	0.06 ± 0.01	0.07 ± 0.01	0.46 ± 0.06	>2	>2	>2
15	0.33 ± 0.02	0.24 ± 0.03	>2	>2	>2	>2
19	0.33 ± 0.02	0.26 ± 0.02	>2	>2	>2	>2
20	0.11 ± 0.01	0.07 ± 0.01	1.0 ± 0.08	>2	>2	>2
21	0.24 ± 0.02	0.21 ± 0.02	>2	>2	>2	>2
22	0.13 ± 0.10	0.07 ± 0.02	>2	>2	>2	>2
26	0.02 ± 0.01	0.03 ± 0.01	0.57 ± 0.06	>2	>2	>2
doxorubicin	0.02 ± 0.01	0.02 ± 0.01	0.02 ± 0.01	0.09 ± 0.01	0.25 ± 0.01	0.15 ± 0.03

^aResults are expressed as IC₅₀ values in μM. Doxorubicin and DMSO were used as positive and negative controls. Withanolides 1–5, 7, 10, 14, 16–18, and 23–25 had no activity up to 5.0 μM.

^bKey: LNCaP = androgen-sensitive human prostate adenocarcinoma; 22Rv1 = androgen-resistant human prostate adenocarcinoma; ACHN = human renal adenocarcinoma; M14 = human melanoma; SK-MEL-28= human melanoma; HFF = human foreskin fibroblast.