Figure 8.

ZEB1 silencing reduces migration and invasion in thyroid cancer cells. (A) Analysis of ZEB1 silencing in K1 cells (left panel). Total protein extracts were analyzed by Western blot to assess the protein levels of ZEB1 after 48 h of siZEB1-1 and siZEB1-2 transfection. Actin was used as a loading control. A representative Western blot analysis of three independent experiments is shown. Quantification of migration rates in ZEB1-silenced cells vs control cells is shown in the right panel. (B) Invasion ability of ZEB1-silenced K1 cells. Left: representative images of the lower chamber (invading cells). Right: percentage of invasiveness relative to siControl cells. Values represent mean ± s.e.m. from four independent experiments **P < 0.01. (C) Upper panel: Representative Western blot of three independent experiments showing ZEB1 protein levels in thyroid cell lines. Actin was used as a loading control. Lower panel: ZEB1 protein expression in thyroid cell lines normalized to expression of actin. Values represent mean ± s.e.m. of three independent experiments. *P < 0.05, **P < 0.001, ****P < 0.0001 vs NThyORI control cells. A full color version of this figure is available at https://doi.org/10.1530/ERC-19-0156.