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. Author manuscript; available in PMC: 2020 Jan 31.
Published in final edited form as: Methods Mol Biol. 2009;523:125–140. doi: 10.1007/978-1-59745-190-1_9

Fig. 9.2.

Fig. 9.2.

Calibration of the degree of phosphorylated H2AX present in relation to the amount of UVA laser intensity used for microirradiation. Several groups of wild-type MEFs were exposed to different amounts of 364 nm laser intensity. A single rectangular ROI was placed on each cell in a group and the cells subsequently microirradiated in that ROI. Confocal images of the resulting phosphorylated H2AX (γ-H2AX, left column) are shown. The corresponding DIC images are also shown (right column). Scale bar, 20 μm.