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. 2020 Jan 1;10(4):1649–1677. doi: 10.7150/thno.40919

Figure 11.

Figure 11

RNAi-mediated knocking-down of AMPK impairs myelin degradation, axonal regeneration and remyelination. (A) Co-immunostaining with anti-MPZ (green) and anti-GFAP (red) antibodies in injured sciatic nerve at day 5. Nuclei were blue (DAPI). (B) The positive MPZ areas in each group were calculated. Data are presented as mean ± SEM; n = 3 rats per group. MPZ F(4, 10) = 12.23, *PNGF vs LV-AMPK = 0.020, **PNGF vs LV-LC3β = 0.0087. (C) Double-immunostaining for MBP (red)/NF-200 (green) and TEM images of sections from the injured sciatic nerve in each group rats at 14 days. Nuclei were blue (DAPI). (D-G) Analysis of NF-200 and MBP positive staining, numbers of myelin sheaths and G-ratio in each group. Data are presented as mean ± SEM; n = 3 rats per group. NF-200 F(4, 10) = 9.77, *PNGF vs LV-AMPK = 0.015, *PNGF vs LV-LC3β = 0.030; MBP F(4, 10) = 12.23, *PNGF vs LV-AMPK = 0.020, *PNGF vs LV-LC3β = 0.017; myelin numbers F(4, 10) = 9.48, *PNGF vs LV-AMPK = 0.014, *PNGF vs LV-LC3β = 0.022; G-ratio F(4, 10) = 10.45, *PNGF vs LV-AMPK = 0.013, *PNGF vs LV-LC3β = 0.017. Significance was determined with the unpaired t-test with Welch's correction.