Figure 13.

Autophagy inhibition suppresses nerve regeneration. (A, B) Electron micrographs and co-immunofluorescence of NF-200 (green) and MBP (red) analysis were performed in the four groups at 14 days after PNI. Nuclei are stained with DAPI (blue). (C-F) Statistical analysis of the G-ratio, distribution of myelin thickness, and NF-200 and MBP positive staining areas on the proximal nerve lesions in each group. Data are presented as the mean ± SEM; n = 3 rats per group. G-ratio F_{(3, 8)} = 8.23, ^*P_{PNI vs NGF} = 0.039, ^*P_{NGF vs NGF+3-MA} = 0.040, P_{PNI vs 3-MA} = 0.49 (n.s); NF-200 F_{(3, 8)} = 10.17, ^*P_{PNI vs NGF} = 0.042, ^*P_{NGF vs NGF+3-MA} = 0.048, P_{PNI vs 3-MA} = 0.35 (n.s); MBP F_{(3, 8)} = 13.91, ^**P_{PNI vs NGF} = 0.005, ^*P_{NGF vs NGF+3-MA} = 0.039, P_{PNI vs 3-MA} = 0.058 (n.s).