Figure 8.

Inhibition of p75^NTR reduces NGF-medicated autophagy and delays myelin clearance and axonal remyelination after sciatic nerve crush injury. (A) Western blots of p75^NGF in the NGF and NGF+TAT-Pep 5 groups 5 days after injury. (B) Changes in the p-AMPK/AMPK, p-p70s6k/p70s6k and p-mTOR/mTOR ratios in each injured nerve. (C) Immunoblotting and quantitative analysis of ATG-7, ATG-5, Beclin-1 P62 and LC3 in the two groups. (D) The protein expression of MBP and MPZ in each group at 5 days post-injury. Quantitative analysis and statistical difference of western blotting results in these two groups were listed in table 2. Data are the mean values ± SEM; n = 3 or 4 independent experiments. (E, F) Immunofluorescence images and quantification of MPZ and GFAP signals in ipsilateral nerves 5 days after injury in NGF or NGF+TAT-Pep treatment rats. Data are the mean values ± SEM; n = 3 rats per group. MPZ ^*P_{NGF vs NGF+TAT-Pep5} = 0.043, t = 3.205, d.f. = 4. (G) Representative TEM images of 14 day sections in each experimental group. Scale bar: 20 μm (HE), 2 μm (TEM) and 25 μm (Immunofluorescence). (H-I) Quantification of the distribution of myelin thickness, G-ratio. Data are represented as the means ± SEM; n = 3 rats per group. G-ratio ^*P_{NGF vs NGF+TAT-Pep5} = 0.043, t = 2.938, d.f. = 4.