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. 2020 Jan 1;10(4):1758–1776. doi: 10.7150/thno.39013

Figure 6.

Figure 6

DOT1L acetylation protects DOT1L from degradation via preventing the interaction between RNF8 and DOT1L. (A) Co-IPs were performed to detect the interaction between RNF8 and DOT1L(WT), DOT1L(K358Q) or DOT1L(K358R) in HCT116 cells. (B) HCT116 cells were transfected with a non-specific shRNA negative control (shNC) or an RNF8 siRNA for 24 h followed by transfection with Flag-DOT1L(WT) and Flag-DOT1L(K358R) for 48 h. The whole cell lysates were analyzed by western blotting with the indicated antibodies. (C) HCT116 cells were transfected with pcDNA or HA-CBP, and then subjected to immunoprecipitation (IP) and western blotting with the indicated antibodies. (D) HCT116 cells were transfected with non-specific shNC or CBP siRNAs, and the proteins were analyzed by western blotting with the indicated antibodies. (E) HCT116 cells were transfected with shNC or RNF8 siRNA for 24 h, and then transfected with HA-CBP for 48 h. The whole cell lysates were extracted and analyzed by western blotting with the indicated antibodies.