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. 2020 Jan 1;10(4):1798–1813. doi: 10.7150/thno.38210

Figure 6.

Figure 6

ZNF750 binds to the promoter of SNAI1 and inhibits its activity in ESCC cells. (A) The putative binding sites in the promoter regions of SNAI1, SNAI2 and SNAI3. (B) ChIP showed ZNF750 binds to the promoter of SNAI1 and SNAI2 in ESCC cells. (C) ZNF750 inhibited the promoter activity of SNAI1 through directly binding to the promoter region from -417 to -410 but didn't affect the promoter activity of SNAI2. (D) ZNF750 rescue significantly decreased the reporter activity of pGL3-SNAI1-promoter (-417 to -410) induced by ZNF750 knockdown. (E) SNAI1 knockdown in KYSE140 cell line with ZNF750 knockdown; The protein levels of ZNF750 and SNAI1 were detected by Western blot. β-actin was used as a loading control. (F) SNAI1 knockdown inhibited the proliferation induced by ZNF750 knockdown. (G) SNAI1 knockdown inhibited the colony formation induced by ZNF750 knockdown. (H) SNAI1 knockdown inhibited the migration induced by ZNF750 knockdown. (I) SNAI1 knockdown inhibited the invasion induced by ZNF750 knockdown. * P < 0.05, ** P < 0.01, *** P < 0.001, ns: Non-significant.