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. 2020 Jan 1;10(4):1833–1848. doi: 10.7150/thno.39814

Figure 3.

Figure 3

UCH-L1 regulates the transcription of ERα gene via EGFR pathway. (A) MCF-7 or T47D cells were transfected with a control plasmid or a myc-his-UCH-L1 plasmid. (B and C) HCC1806 or BT549 cells were transfected with a non-targeting siRNA or UCH-L1 siRNAs for 72h (B), or were treated with 10 μM LDN for 24h (C). The ERα mRNA level was analyzed by real-time PCR. (D) MCF-7 cells were transfected with a control plasmid or a Flag-EGFR plasmid. The mRNA level of ERα was measured by real-time PCR. The expressions of EGFR and ERα were measured by western blot. β-actin was used as a loading control. Results shown are Mean ± s.d., n=3. ∗, p <0.05; ∗∗, p <0.01. (E) MCF-7/AdrR cells were transfected with a non-targeting siRNA or an UCH-L1 siRNA, followed by transfection with a Flag-EGFR expression plasmid. (F) MCF-7 cells overexpressing UCH-L1 were transfected with a non-targeting siRNA or an EGFR siRNA. The mRNA level of ERα was measured by real-time PCR. The expressions of UCH-L1, ERα and EGFR were measured by western blot. β-actin was used as a loading control.