Figure 7.

AuNCs promote the nuclear accumulation of Nrf2. U251N cells were treated for the times indicated with vehicle, 300 μM of H₂O₂, 10 μM of Au₁₅SG₁₃ or 10 μM Au₁₅PEG for the times indicated. Cells were processed for immunocytochemistry with antibodies against Nrf2. Nuclei were detected with Hoechst 33342 and F-actin stained with phalloidin. After incubation for 10, 30 or 60 minutes, Nrf2 nuclear translocation was quantified by assessing Nrf2 fluorescence in the nucleus and cytosol. Three independent experiments were conducted and a total of 120 cells were analyzed. The graph shows average ± SEM; *, p<0.05; **, p< 0.01; ***, p<0.001.