Figure 7.
(A) Representative microscopic images of gastrocnemius muscle collected from ischemic hindlimb before (control), and at 3, 7, and 14 days after the surgical ligation of right femoral artery simultaneously incubated with an antibody against αV integrin (left) and fluorescent FITC-labeled analogue of NC100692 (middle). Right column represents a result of merging of two-color channels (αV integrin and fluorescent analogue of NC100692). (B) Quantitative analysis of co-localization between αV integrin and fluorescent analogue of NC100692 in ischemic gastrocnemius muscle samples collected before (control) and at 3, 7, and 14 days after the surgical ligation of right femoral artery. Values are presented as percentage of cells expressing αV integrin co-localized with fluorescent FITC-labeled NC100692 analogue (solid bars). ∗P < 0.05 vs. control. #P < 0.05 vs. 14 days after ligation.