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. 2020 Jan 24;9:1473. doi: 10.3389/fonc.2019.01473

Figure 4.

Figure 4

FGFR3 is a direct target gene of miR-99a. (A) The putative binding site of miR-99a in the FGFR3 3′ UTR, and the mutated 3′ UTR. (B) qRT-PCR of FGFR3 in MCF-7 cells transfected with miR-99a or the scramble control sequence. (C) The luciferase activity of 293T cells was detected after the pmirGLO plasmids with WT or Mut FGFR3 3′ UTR genes were co-transfected with miR-NC or miR-99a mimics. (D) FGFR3 silencing inhibited proliferation of MCF-7 cells. MCF-7 cells were transfected FGFR3 siRNA (siR-FGFR3) or the scramble control siRNA. Cell viability was determined by CCK8 assay in MCF-7 cells. (E) FGFR3 silencing inhibited migration and invasion of MCF-7 cells. Transwell migration and matrigel invasion assays on MCF-7 cells were transfected FGFR3 siRNA or the scramble control siRNA. Means of three independent experiments ± SEM were shown (**P < 0.01).