Figure 2.

Representative electron micrographs of one nonresistant (A, B, C, D, E and F) and one resistant clone (G, H, I, J, K and L), treated for 72 h with either vehicle control (A, B, C, G, H and I) or 50 µM mitotane (D, E, F, J, K and L). Mitotane treatment causes mitochondrial swelling, loss of mitochondrial cristae and irregular nuclear shapes in nonresistant, but not in resistant cells. N: nuclei; *: mitochondria; LD: lipid droplet. Lines: 5 µm (A, D, G and J); 2.5 µM (B, E, H and K); 1.25 µm (C, F, I and L).